P-352 Evaluation of CD25 and CD69 activation markers expression on peripheral blood cells subpopulations in endometriosis patients

Abstract Study question We aim to determine the percentages of CD25-positive and CD69-positive lymphocytes in peripheral blood endometriosis (EMS) patients. Summary answer Higher expressing CD69 CD25 markers EMS their correlation with severity disease, indicate persistent activation What is known already Exposure antigens causes appear on surface lymphocytes. Among them, we can distinguish early late markers. The earliest one CD69, which expressed upon by TCR receptor. plays a role proliferation survival activated T moderate marker, considered most important marker cellular response activation. significance expression cells has not yet been determined. Increased various lymphocyte subsets peritoneal fluid reported. design, size, duration Within this prospective study between January 2016 August 2018 immune diagnostics number patients (firstly diagnosed during laparoscopy confirmed histopatologically) non-EMS controls were performed using flow cytometry analysis. Total 74 subjects included study. All signed written informed consent before enrollment Participants/materials, setting, methods enrolled 54 previously untreated 20 healthy age-matched controls. Peripheral was collected from control assess immunophenotype measure Cd25 several subtypes B Diagnosis assessment stage established rASRM score. Differences statistically significant p < 0.05. Main results chance Significantly higher antigen found both CD3+ T, CD4+ CD8+ as well CD19+ cells. In each studied groups, level 0.001 obtained. There weak positive percentage CD4+CD25+ (R = 0.357; 0.008) negative correlations CD3+CD69+ −0.554; 0.001), CD4+CD69 + −0.554, 0.001) CD8+CD69 −0.553, 0.001). markers, suffering accompanying clinical symptoms also evaluated. n group accompanied infertility or pelvic pain, no statistical differences observed. only condition coexisting different adhesion disease - significantly lower CD3+CD69 (p 0.002), 0.002) 0.002). Limitations, reasons for caution Small sample size an limitation addition, evaluating immunological analysis serum samples does let us drive any conclusions local changes lesions. Wider implications findings Clinical interpretation changed could be useful mechanisms activation, tolerance imbalance involved pathogenesis endometriosis. Further studies understand underlying should considered. Trial registration applicable